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KMID : 1094720110160010120
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 1 p.120 ~ p.126
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Diarylheotanoid from Alnus hirsuta improves glucose metabolism via insulin signal transduction in human hepatocarcinoma (HepG2) cells
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Hu Weicheng
Wang Myeong-Hyeon
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Abstract
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Alnus hirsuta is a deciduous, broad-leaved tree that thrives in damp locations, and the bark of the Alnus species has been used in traditional oriental medicine as a remedy for hemorrhage, fever, diarrhea, and alcoholism. Two diarylheptanoids, 1,7-bis-(3,4-dihydroxyphenyl)-heptane-3-one-5-O-β-D-xylopyranoside (Compound I) and 1,7-bis-(3,4-dihydroxyphenyl)-heptane-5-O-β-D-xylopyranoside (Compound II), were isolated from the stem bark of A. hirsuta. In the present study, the investigations of two diarylheptanoids on insulin signaling were performed in insulin-responsive human hepatocarcinoma (HepG2) cells co-treated with high glucose. As the results, the insulin metabolic effect of glucose uptake was inhibited by high glucose. However, treatment of Compound I enhanced the glucose uptake ability in HepG2 cells, whereas, Compound II did not affect glucose incorporation noticeably. In addition, we demonstrated that treatment of HepG2 cells with Compound I improved the mRNA expression level of insulin receptor (IR) and insulin receptor substrate-1 (IRS-1).
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KEYWORD
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antidiabetic, Alnus hirsuta, diarylheptanoid, insulin receptor
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